About

It was developed by Frederick Sanger and his colleagues in the late 1970s. This technique involves DNA synthesis using modified versions of the four nucleotides (A, T, G, and C) that are labeled with fluorescent or radioactive tags. The DNA fragments are then separated by size using gel electrophoresis, and the sequence is read based on the order of the labeled nucleotides. Sanger sequencing has been widely used in genetics research and DNA diagnostics, allowing scientists to determine the order of nucleotides in a DNA molecule accurately. It has been instrumental in various fields such as genome mapping, gene identification, and forensic analysis. While Sanger sequencing was the primary method used for DNA sequencing for several decades, it has now largely been replaced by more high-throughput and cost-effective techniques such as next-generation sequencing. However, Sanger sequencing is still used in specific applications that require high accuracy and long read lengths, such as validation studies and confirmation of genetic variants.