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High-performance liquid chromatography

High-performance liquid chromatography (HPLC) is a powerful analytical technique used in chemistry and biochemistry to separate, identify, and quantify various components in a mixture. It relies on the principles of liquid chromatography, utilizing a mobile phase (liquid) and a stationary phase (solid) to separate compounds based on their interactions with the stationary phase.

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It relies on the principles of liquid chromatography, utilizing a mobile phase (liquid) and a stationary phase (solid) to separate compounds based on their interactions with the stationary phase. HPLC offers high resolution, sensitivity, and versatility, making it widely employed in a range of applications such as pharmaceutical analysis, forensic analysis, environmental monitoring, and food analysis. The Wikipedia page on HPLC provides a comprehensive overview of the technique, including its history, basic principles, instrumentation, types of HPLC columns, and different modes of HPLC separation. It also covers key aspects like sample preparation, detection methods, and data analysis. Moreover, the page delves into the various applications of HPLC and discusses its advantages and limitations. The content incorporates references to scientific literature and includes links to related topics, allowing readers to explore the subject in more depth.

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